Byung Kim
University of Massachusetts Amherst, USA
Title: Detection of pancreatic cancer biomarkers using a SERS-based immunoassay using gold nanoparticles and nanoshells
Biography
Biography: Byung Kim
Abstract
Early diagnosis of pancreatic cancer (PC) is critical to reducing the mortality rate of this disease. Current biological analysis approaches cannot robustly detect several low abundance PC biomarkers in sera, limiting the clinical application of these biomarkers. Enzyme-linked immunosorbent assay and radioimmunoassay are two common platforms for detection of biomarkers; however, they suffer from some limitations. This presentation investigates a novel system for multiplex detection of pancreatic biomarkers CA19-9, MMP7, Mesothelin and MUC4 in sera samples with high sensitivity using surface-enhanced Raman spectroscopy. The gold nanoshell showed better Raman intensity compared to that of the gold nanoparticles. Measuring the Raman signals of these biomarkers in PC patients, pancreatitis patients, and healthy individuals reveal the unique expression pattern of these markers in PC patients, suggesting the great potential of using this approach for early diagnostics of PCs. Advantages and feasibility of performing this platform on an integrated microfluidic device also will be discussed.